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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in several tumors, which leads to chromosome instability, and thus might contribute to tumorigenesis. This protein was discovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant role in selling cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up claimed but comprehensive nucleotide sequence info is not really but readily available.
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Blocking peptides are peptides that bind especially to your focus on antibody and block antibody binding. These peptide generally has the epitope acknowledged by the antibody.
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This guidebook outlines even more possible optimization of cellular and immunoassay parameters to guarantee the absolute best final results are...
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This tutorial outlines additional doable optimization of cellular and immunoassay parameters to ensure the absolute best success are...
Antibodies certain to the blocking peptide no longer bind on the epitope to the target protein. By comparing the staining in the blocked antibody vs . the antibody by yourself, one can see which staining is precise.
Concentrate on Data Cyclin E belongs towards the very conserved cyclin household, whose associates are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show distinctive expression and degradation styles which lead on the temporal coordination of each mitotic occasion. Cyclin E kinds a complex with and capabilities as a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates with the G1-S section boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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